探讨在高温胁迫时幼苗的各项生理指标变化
以枣树幼苗为试验材料，对其幼苗进行模拟高温胁迫处理，研究模拟高温胁迫条件下，枣树幼苗细胞内叶片含水量、POD、丙二醛、脯氨酸的变化趋势，为高温条件下各地区枣种的栽培提供理论依据。
Jujube seedlings as experimental material, to handle high temperature stress of the seedling, the simulation under the condition of high temperature stress, jujube seedling leaf water content in the cell, the change of POD, mda, proline, jujube for high temperature under the condition of the region to provide theoretical basis for the cultivation.
1 材料和方法
1 materials and methods
1.1材料
1.1 material
取长势一致的1年生营养苗，消毒洗净之后，于完全培养液中进行培养，观察其生长状况，并用AP9500D潜水泵每天通气1 h。温棚条件均为：温度22～35 ℃，相对湿度65％～75％，选用自然光照并用遮阴网遮阴。
Looks like the take consistent nutritional seedlings, 1 year after cleaning disinfection, to cultivate a culture completely, observe its growth conditions, using
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AP9500D submersible pump ventilation 1 h every day. The greenhouse conditions are: temperature 22 ~ 35 ℃, relative humidity 65% ~ 75%, and use natural light and shade shade net.
1.2方法
1.2 method
1.2.1试验处理 取在培养液中培养5 d的1年生苗分别置于4个不同的恒温培养箱中，恒温培养箱温度分别设置为20，25，30，35 ℃。在恒温培养箱中模拟高温胁迫处理48 h，以20 ℃作为对照组，采集新鲜叶片进行相关指标的测定，重复3次。
1.2.1 test treatment take in nutrient solution culture 5 d 1 year seedlings, respectively in four different constant temperature incubator, constant temperature incubator temperature set respectively for 20,25,30,35 ℃. Handle high temperature stress in the constant temperature incubator 48 h, 20 ℃ as control group, freshly collected the blade to determine the relevant indicators, repeat 3 times.
1.2.2 生理生化指标测定叶片相对含水量参考张志良的方法测定；细胞膜相对透性采用电导法测定，丙二醛测定采用三氯乙酸-硫代巴比妥酸法。
1.2.2, physiological and biochemical indicators to determine the method for determining leaf relative water content of the reference \"volatility-smile warrant hedging; Cell membrane relative permeability using conductance method,
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determination of malondialdehyde the trichloroacetic acid - thio barbituric acid method.
2结果与分析
2 the results and analysis
1高温胁迫对枣树幼苗电解质渗透率的影响
1 high temperature stress on jujube seedlings electrolyte permeability
高温胁迫对枣树幼苗电解质渗透率的影响如图1所示，与对照组相比，25 ℃处理的电解质渗透率的变化幅度不显着，但30 ℃和35 ℃处理后，电解质渗透率变化明显，其中，在35 ℃处理下的枣树幼苗，其电解质渗透率由开始的20%上升至60%以上。30 ℃处理下的枣树幼苗叶片的电解质渗透率也高于对照组。可见，高温对枣树幼苗的电解质渗透率有着较为显着的影响。 2模拟高温胁迫对枣树叶片中丙二醛（MDA）含量的影响
High temperature stress on jujube seedlings as shown in figure 1, the influence of electrolyte permeability compared with the control group, 25 ℃ treatment of electrolyte permeability change is not significant, but the 30 ℃ and 35 ℃ after processing, the electrolyte permeability changes obviously, among them, the jujube seedlings under 35 ℃ treatment, its electrolyte permeability rose to 60% from 20% to above. 30 ℃ under the treatment of jujube seedlings leaf electrolyte
permeability
is
higher
than
the
control
group.
Visible,
high-temperature electrolyte permeability of jujube seedlings had a significant
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impact. 2 simulation of high temperature stress on in Chinese jujube leaf malondialdehyde (MDA) content
高温胁迫对枣树幼苗丙二醛含量的影响如图2所示，通过图2可以看出，丙二醛随着温度胁迫的上升，有着明显的上升趋势，并且温度胁迫处理的时间越久，上升的越明显，在处理12 h后，各试验组丙二醛的含量与胁迫温度呈线性关系，在胁迫处理48 h后，35 ℃处理的试验组较对照组出现了显着的上升，增幅达300%。可见胁迫时间越久、温度越高，丙二醛含量上升的越快。 3模拟高温胁迫对枣树幼苗叶片含水量的影响
High temperature stress on jujube seedlings malondialdehyde content as shown in figure 2, the influence of by figure 2 can see, with malondialdehyde rise in temperature stress, a clear upward trend, the longer treatment time and temperature stress, the rising, the more obvious, in 12 h after treatment, the treatment group of malondialdehyde content and stress of a linear relationship between temperature, stress processing after 48 h, 35 ℃ in treatment group than the control group appeared a significant rise, growth of 300%. Visible stress the longer, the higher the temperature, content of malondialdehyde rise faster. 3 high temperature stress effects on jujube seedling leaf water content
高温胁迫对枣树幼苗叶片相对含水量的影响如图3所示，植物叶片的相对含水量对维持植物的正常生理特性有着十分重要的意义 。由图可知，叶片相对含水量有降低的趋势，但降幅不明显，即使是35 ℃胁迫下，处理了48 h后，叶片相对含水量也只下降了23%，这与枣树幼苗上厚厚的角质有关。25 ℃试验组，叶片相对含水量较对照组基本没有发生变化，可见枣树幼苗的叶片相对含水量受高温胁迫而减少的效果不明显。
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High temperature stress on jujube seedling leaf relative water content as shown in figure 3, the influence of the relative water content of plant leaves to maintaining normal physiological characteristics of plants is of great significance. The figure shows that leaf relative water content has a tendency to reduce, but the decline is not obvious, even under 35 ℃ stress, processing after 48 h, fell by 23% leaf relative water content, it has to do with thick cutin on jujube seedlings. 25 ℃ group, leaf relative water content than the control group the basic no change, visible leaf relative water content of jujube seedlings under high temperature stress and reduce the effect is not obvious.
